One woman came forward to accuse the disgraced financier Jeffrey Epstein of sexually abusing her at the age of 15, and asked Prince Andrew to share information about his former friend.
The new accuser, identified as "Jane Doe 15", told reporters that he suffered a "prolonged and cruel sexual assault".
She is the latest in more than a dozen women to prosecute Epstein's assets for alleged sexual abuse.
This is amid controversy over Prince Andrew's ties to the sex offender.
In a BBC interview on Sunday, Prince Andrew pressed his friendship with Epstein, and a Virginia Giuffre accusation that he had sex with her at Epstein's house when Giuffre was 17 years old.
Prince Andrew denied having sex with Giuffre, but the interview created a wave of criticism directed at royalty. Accounting firm KPMG announced on Monday that it would not renew its sponsorship of the prince's entrepreneurial initiative, Pitch @ Palace.
A new accuser
Jane Doe 15, now 31, gave a press conference in Los Angeles on Monday with her lawyer, Gloria Allred. She accused Epstein of abusing her on his New Mexico farm at the age of 15, after meeting his secretary during a 2004 school trip to New York.
Under the lawsuit, the secretary said Epstein was interested in helping girls from poorer backgrounds. She was flown by private jet to her New Mexico ranch with several other girls, she said. She claims he told her to have a massage and then sexually assaulted her.
"Epstein took my sexual innocence," she said during the press conference.
Epstein, 66, hanged himself in prison in August while awaiting trial on federal charges of sex trafficking. He has been accused of sexually abusing dozens of girls, some 14 years old. He pleaded not guilty.
Jane Doe 15 did not accuse Prince Andrew of any wrongdoing, but asked him to provide further information. "Prince Andrew and any others close to Epstein must make a sworn statement as to what information they have," she said.
Allred said he had asked Prince Andrew to "meet voluntarily with the FBI and prosecutors investigating this criminal case in New York, and also to give his sworn statement."