The construction of the 401 will result in some significant deviations for drivers from Sunday night.
The west ramp for Highway 8 and King Street East will be closed from 10pm.
This means that drivers will only have the option of leaving on Hespeler Road or Homer Watson Boulevard.
The left and center lanes of the east and west lanes will also be closing on Monday between Hespeler Road and Highway 8.
These reductions will begin at 8pm. and continue until Thursday at 6 in the morning.
Drivers are being advised to plan an alternative route.